Key features and details
- Rabbit polyclonal to SLC12A1/NKCC2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC12A1/NKCC2 antibody
See all SLC12A1/NKCC2 primary antibodies
DescriptionRabbit polyclonal to SLC12A1/NKCC2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Orangutan
Synthetic peptide corresponding to Human SLC12A1/NKCC2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human kidney tissue lysate.
This product was previously labelled as SLC12A1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).|
FunctionElectrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
Tissue specificityKidney specific.
Involvement in diseaseDefects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
Sequence similaritiesBelongs to the SLC12A transporter family.
- Information by UniProt
- BSC1 antibody
- Bumetanide sensitive sodium 3 antibody
- Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
Anti-SLC12A1/NKCC2 antibody (ab91098) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
SLC12A1/NKCC2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab91098 has not yet been referenced specifically in any publications.