Overview

  • Product name

    Anti-SLC12A1/NKCC2 antibody
    See all SLC12A1/NKCC2 primary antibodies
  • Description

    Rabbit polyclonal to SLC12A1/NKCC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SLC12A1/NKCC2 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101074)

  • Positive control

    • This antibody gave a positive signal in Human kidney tissue lysate.
  • General notes

     This product was previously labelled as SLC12A1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

Target

  • Function

    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificity

    Kidney specific.
  • Involvement in disease

    Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • Sequence similarities

    Belongs to the SLC12A transporter family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSC1 antibody
    • Bumetanide sensitive sodium 3 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
    • Kidney specific Na K Cl symporter antibody
    • Kidney-specific Na-K-Cl symporter antibody
    • MGC48843 antibody
    • Na K 2Cl cotransporter antibody
    • NKCC2 antibody
    • potassiumchloride cotransporter 2 antibody
    • S12A1_HUMAN antibody
    • Slc12a1 antibody
    • sodium potassium chloride cotransporter 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
    • Solute carrier family 12 member 1 antibody
    see all

Images

  • Anti-SLC12A1/NKCC2 antibody (ab91098) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    SLC12A1/NKCC2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

ab91098 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab91098.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up