Overview

  • Product name

    Anti-SLC12A1/NKCC2 antibody [EPR11842]
    See all SLC12A1/NKCC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2
  • Host species

    Rabbit
  • Specificity

    The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so ab171747 may cross react with SLC12A2. We haven't performed any laboratory testing about this.

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SLC12A1/NKCC2. The exact sequence is proprietary.
    Database link: Q13621

  • Positive control

    • 293T, Human fetal kidney, K562 and WI-38 cell lysates; permeabilized K562 cells.
  • General notes

     This product was previously labelled as SLC12A1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11842
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 121 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
    • Tissue specificity

      Kidney specific.
    • Involvement in disease

      Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
    • Sequence similarities

      Belongs to the SLC12A transporter family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BSC1 antibody
      • Bumetanide sensitive sodium 3 antibody
      • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
      • Kidney specific Na K Cl symporter antibody
      • Kidney-specific Na-K-Cl symporter antibody
      • MGC48843 antibody
      • Na K 2Cl cotransporter antibody
      • NKCC2 antibody
      • potassiumchloride cotransporter 2 antibody
      • S12A1_HUMAN antibody
      • Slc12a1 antibody
      • sodium potassium chloride cotransporter 2 antibody
      • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
      • Solute carrier family 12 member 1 antibody
      see all

    Images

    • All lanes : Anti-SLC12A1/NKCC2 antibody [EPR11842] (ab171747) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : Human fetal kidney lysates
      Lane 3 : K562 cell lysates
      Lane 4 : WI-38 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 121 kDa

    • Flow cytometric analysis of permeabilized K562 cells labeling SLC12A1/NKCC2 with ab171747 at 1/100 dilution (red), compared with a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Duan XP  et al. Norepinephrine-Induced Stimulation of Kir4.1/Kir5.1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule. Hypertension 73:112-120 (2019). Read more (PubMed: 30571558) »
    • Teng F  et al. Treatment with an SLC12A1 antagonist inhibits tumorigenesis in a subset of hepatocellular carcinomas. Oncotarget 7:53571-53582 (2016). IHC . Read more (PubMed: 27447551) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171747.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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