Recombinant
RabMAb

Recombinant Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free (ab213653)

Overview

  • Product name

    Anti-SLC12A1/NKCC2 antibody [EPR11842] - BSA and Azide free
    See all SLC12A1/NKCC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11842] to SLC12A1/NKCC2 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The homology between SLC12A1/NKCC2 and SLC12A2 is 67%, so this antibody may cross react with SLC12A2. We haven't performed any laboratory testing about this.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SLC12A1/NKCC2. The exact sequence is proprietary.
    Database link: Q13621

  • Positive control

    • 293T, Human fetal kidney, K562 and WI-38 cell lysates; permeabilized K562 cells.
  • General notes

    Ab213653 is the carrier-free version of ab171747. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab213653 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11842
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 121 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
    • Tissue specificity

      Kidney specific.
    • Involvement in disease

      Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
    • Sequence similarities

      Belongs to the SLC12A transporter family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BSC1 antibody
      • Bumetanide sensitive sodium 3 antibody
      • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
      • Kidney specific Na K Cl symporter antibody
      • Kidney-specific Na-K-Cl symporter antibody
      • MGC48843 antibody
      • Na K 2Cl cotransporter antibody
      • NKCC2 antibody
      • potassiumchloride cotransporter 2 antibody
      • S12A1_HUMAN antibody
      • Slc12a1 antibody
      • sodium potassium chloride cotransporter 2 antibody
      • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
      • Solute carrier family 12 member 1 antibody
      see all

    References

    ab213653 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab213653.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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