Key features and details
- Rabbit polyclonal to SLC12A1/NKCC2 - N-terminal
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-SLC12A1/NKCC2 antibody - N-terminal
See all SLC12A1/NKCC2 primary antibodies
DescriptionRabbit polyclonal to SLC12A1/NKCC2 - N-terminal
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Human
- Rat kidney tissue and lysate
This product was previously labelled as SLC12A1
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 121 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionElectrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
Tissue specificityKidney specific.
Involvement in diseaseDefects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
Sequence similaritiesBelongs to the SLC12A transporter family.
- Information by UniProt
- BSC1 antibody
- Bumetanide sensitive sodium 3 antibody
- Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
Anti-SLC12A1/NKCC2 antibody - N-terminal (ab191315) at 0.5 µg/ml + rat kidney tissue lysate
Predicted band size: 121 kDa
Immunohistochemical analysis of paraffin-embedded rat kidney tissue, labeling SLC12A1/NKCC2 with ab191315 at 1 μg/ml.
Immunohistochemical analysis of frozen rat kidney tissue, labeling SLC12A1/NKCC2 with ab191315 at 1 μg/ml.
ab191315 has been referenced in 2 publications.
- Mohr J et al. IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia. FASEB J N/A:fj201801875RR (2019). PubMed: 30721632
- Sasaki S et al. A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle. BMC Genomics 17:724 (2016). IHC-Fr ; Cow . PubMed: 27613513