Overview

  • Product name

    Anti-SLC12A1/NKCC2 antibody - N-terminal
    See all SLC12A1/NKCC2 primary antibodies
  • Description

    Rabbit polyclonal to SLC12A1/NKCC2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human SLC12A1/NKCC2 aa 125-139 (N terminal).
    Sequence:

    KVNRPSLLEIHEQLA


    Database link: Q13621

  • Positive control

    • Rat kidney tissue and lysate
  • General notes

     This product was previously labelled as SLC12A1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 121 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificity

    Kidney specific.
  • Involvement in disease

    Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • Sequence similarities

    Belongs to the SLC12A transporter family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSC1 antibody
    • Bumetanide sensitive sodium 3 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
    • Kidney specific Na K Cl symporter antibody
    • Kidney-specific Na-K-Cl symporter antibody
    • MGC48843 antibody
    • Na K 2Cl cotransporter antibody
    • NKCC2 antibody
    • potassiumchloride cotransporter 2 antibody
    • S12A1_HUMAN antibody
    • Slc12a1 antibody
    • sodium potassium chloride cotransporter 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
    • Solute carrier family 12 member 1 antibody
    see all

Images

  • Anti-SLC12A1/NKCC2 antibody - N-terminal (ab191315) at 0.5 µg/ml + rat kidney tissue lysate

    Predicted band size: 121 kDa

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue, labeling SLC12A1/NKCC2 with ab191315 at 1 μg/ml.

  • Immunohistochemical analysis of frozen rat kidney tissue, labeling SLC12A1/NKCC2 with ab191315 at 1 μg/ml.

References

This product has been referenced in:

  • Mohr J  et al. IL-6/Smad2 signaling mediates acute kidney injury and regeneration in a murine model of neonatal hyperoxia. FASEB J N/A:fj201801875RR (2019). Read more (PubMed: 30721632) »
  • Sasaki S  et al. A missense mutation in solute carrier family 12, member 1 (SLC12A1) causes hydrallantois in Japanese Black cattle. BMC Genomics 17:724 (2016). IHC-Fr ; Cow . Read more (PubMed: 27613513) »
See all 2 Publications for this product

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