Key features and details
- Rabbit polyclonal to SLC12A6/KCC3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC12A6/KCC3 antibody
See all SLC12A6/KCC3 primary antibodies
DescriptionRabbit polyclonal to SLC12A6/KCC3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SLC12A6/KCC3 aa 25-75. The exact sequence is proprietary. NP_598408.1
Database link: Q9UHW9
- WB: HeLa, Jurkat and HEK-293T whole cell lysate.
This product was previously labelled as SLC12A6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to SLC12A6/KCC3 immobilized on solid support
Our Abpromise guarantee covers the use of ab241348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
FunctionMediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
Tissue specificityHighly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
Involvement in diseaseAgenesis of the corpus callosum, with peripheral neuropathy
Sequence similaritiesBelongs to the SLC12A transporter family.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- ACCPN antibody
- Electroneutral potassium-chloride cotransporter 3 antibody
- Furosemide sensitive KCl cotransporter 3 antibody
All lanes : Anti-SLC12A6/KCC3 antibody (ab241348) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 3 minutes
Prepared using RIPA buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241348 has not yet been referenced specifically in any publications.