Key features and details
- Rabbit polyclonal to SLC12A6/KCC3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC12A6/KCC3 antibody
See all SLC12A6/KCC3 primary antibodies
DescriptionRabbit polyclonal to SLC12A6/KCC3
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human SLC12A6/KCC3 aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following human tissue lysates: Kidney; Skeletal Muscle.
This product was previously labelled as SLC12A6
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab92951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).|
FunctionMediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
Tissue specificityHighly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
Involvement in diseaseAgenesis of the corpus callosum, with peripheral neuropathy
Sequence similaritiesBelongs to the SLC12A transporter family.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- ACCPN antibody
- Electroneutral potassium-chloride cotransporter 3 antibody
- Furosemide sensitive KCl cotransporter 3 antibody
All lanes : Anti-SLC12A6/KCC3 antibody (ab92951) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 122,128 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Blocked with 5% Milk for 1 hour. This antibody should recognise both isoforms of SLC12A6/KCC3. It is possible that we are detecting isoform 1 (128-kDa) in human kidney tissue lysate and isoform 2 (122-kDa) in human skeletal muscle lysate.
ICC/IF image of ab92951 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92951 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 1ug/ml, and in Methanol fixed (100%, 5min) HepG2 cells at 1ug/ml
ab92951 has been referenced in 1 publication.
- Loureiro CA et al. Tyrosine phosphorylation modulates cell surface expression of chloride cotransporters NKCC2 and KCC3. Arch Biochem Biophys 669:61-70 (2019). PubMed: 31145900