Overview

  • Product name

    Anti-SLC12A6/KCC3 antibody - C-terminal
    See all SLC12A6/KCC3 primary antibodies
  • Description

    Rabbit polyclonal to SLC12A6/KCC3 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SLC12A6/KCC3 aa 1060-1080 (C terminal).
    Sequence:

    QKAKSMEGFQDLLNMRPDQSN


    Database link: Q9UHW9

  • Positive control

    • HeLa and HUT102 cell lysate.
  • General notes

     This product was previously labelled as SLC12A6

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 128 kDa.

Target

  • Function

    Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
  • Tissue specificity

    Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
  • Involvement in disease

    Agenesis of the corpus callosum, with peripheral neuropathy
  • Sequence similarities

    Belongs to the SLC12A transporter family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Basolateral cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACCPN antibody
    • Electroneutral potassium-chloride cotransporter 3 antibody
    • Furosemide sensitive KCl cotransporter 3 antibody
    • Gaxp antibody
    • K-Cl cotransporter 3 antibody
    • KCC 3 antibody
    • KCC 3A antibody
    • KCC 3B antibody
    • KCC3 A antibody
    • KCC3 antibody
    • KCC3 B antibody
    • KCC3A antibody
    • KCC3B antibody
    • Potassium chloride cotransporter 3 antibody
    • Potassium chloride cotransporter KCC3a S3 antibody
    • S12A6_HUMAN antibody
    • SLC12 A6 antibody
    • SLC12A 6 antibody
    • SLC12A6 antibody
    • Solute carrier family 12 (potassium/chloride transporters), member 6 antibody
    • Solute carrier family 12 member 6 antibody
    • Solute carrier family 12, member 6 antibody
    see all

Images

  • All lanes : Anti-SLC12A6/KCC3 antibody - C-terminal (ab191451) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HUT102 cell lysate

    Predicted band size: 128 kDa

References

ab191451 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (spleen and Liver)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
5 µg
Specification
spleen and Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C

Dr. Helen Goodluck

Verified customer

Submitted Jan 23 2019

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