Recombinant Anti-SLC14A2 antibody [EPR8620] (ab156589)
![Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)](https://www.abcam.com/ps/products/156/ab156589/Images/SLC14A2-Primary-antibodies-ab156589-1.jpg "Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8620] to SLC14A2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SLC14A2 antibody [EPR8620]
See all SLC14A2 primary antibodies -
Description
Rabbit monoclonal [EPR8620] to SLC14A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SLC14A2 aa 500-600. The exact sequence is proprietary.
Database link: Q15849 -
Positive control
- K562, Human fetal kidney, 293T and TF1 lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8620 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 101 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. -
Tissue specificity
Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. -
Sequence similarities
Belongs to the urea transporter family. -
Cellular localization
Apical cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8170 Human
- Entrez Gene: 27411 Mouse
- Omim: 601611 Human
- SwissProt: Q15849 Human
- SwissProt: Q8R4T9 Mouse
- Unigene: 710927 Human
- Unigene: 44158 Mouse
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Alternative names
- FLJ16167 antibody
- hUT-A6 antibody
- HUT2 antibody
see all
Images
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Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)All lanes : Anti-SLC14A2 antibody [EPR8620] (ab156589) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : 293T lysate
Lane 4 : TF1 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 101 kDa -
![Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)](https://www.abcam.com/ps/products/156/ab156589/Images/SLC14A2-Primary-antibodies-ab156589-4.jpg)
Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)Detection of SLC14A2 by Western Blot of Immunprecipitate.
293T cell lysate pellet, immunoprecipitated using ab156589 at 1/10 dilution. -
![Anti-SLC14A2 antibody [EPR8620] (ab156589)](https://www.abcam.com/ps/products/156/ab156589/Images/ab156589-3-benefits-of-recombinant-antibodies.png)
Anti-SLC14A2 antibody [EPR8620] (ab156589)
Protocols
Datasheets and documents
References (1)
ab156589 has been referenced in 1 publication.
- Serman Y et al. Emerging new role of NFAT5 in inducible nitric oxide synthase in response to hypoxia in mouse embryonic fibroblast cells. Am J Physiol Cell Physiol 317:C31-C38 (2019). PubMed: 31067085
