Anti-SLC16A3/MCT 4 antibody (ab74109)
Key features and details
- Rabbit polyclonal to SLC16A3/MCT 4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC16A3/MCT 4 antibody
See all SLC16A3/MCT 4 primary antibodies -
Description
Rabbit polyclonal to SLC16A3/MCT 4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Extracts from LOVO cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
Target
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Function
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. -
Tissue specificity
Highly expressed in skeletal muscle. -
Sequence similarities
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9123 Human
- Entrez Gene: 80879 Mouse
- Entrez Gene: 80878 Rat
- Omim: 603877 Human
- SwissProt: O15427 Human
- SwissProt: P57787 Mouse
- SwissProt: O35910 Rat
- Unigene: 500761 Human
see all -
Form
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains. -
Alternative names
- MCT 4 antibody
- MCT3 antibody
- MCT4 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab74109 has been referenced in 10 publications.
- Kieran NW et al. MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes. J Neuroinflammation 19:10 (2022). PubMed: 34991629
- Cluntun AA et al. The pyruvate-lactate axis modulates cardiac hypertrophy and heart failure. Cell Metab 33:629-648.e10 (2021). PubMed: 33333007
- Yang S et al. LINC00035 Transcriptional Regulation of SLC16A3 via CEBPB Affects Glycolysis and Cell Apoptosis in Ovarian Cancer. Evid Based Complement Alternat Med 2021:5802082 (2021). PubMed: 34671407
- Zhao D et al. Altered expression of lactate dehydrogenase and monocarboxylate transporter involved in lactate metabolism in broiler wooden breast. Poult Sci 99:11-20 (2020). PubMed: 32416791
- Sun K et al. Oxidized ATM-mediated glycolysis enhancement in breast cancer-associated fibroblasts contributes to tumor invasion through lactate as metabolic coupling. EBioMedicine 41:370-383 (2019). PubMed: 30799198
- Brosel S et al. An auditory brainstem nucleus as a model system for neuronal metabolic demands. Eur J Neurosci 47:222-235 (2018). PubMed: 29205598
- Chatel B et al. Moderate and intense muscular exercises induce marked intramyocellular metabolic acidosis in sickle cell disease mice. J Appl Physiol (1985) 122:1362-1369 (2017). PubMed: 28280108
- England EM et al. Chronic activation of AMP-activated protein kinase increases monocarboxylate transporter 2 and 4 expression in skeletal muscle. J Anim Sci 95:3552-3562 (2017). PubMed: 28805903
- Zawislak A et al. Neuron-derived transthyretin modulates astrocytic glycolysis in hormone-independent manner. Oncotarget 8:106625-106638 (2017). PubMed: 29290976
- Ghorbel MT et al. Changes in renal medulla gene expression in a pre-clinical model of post cardiopulmonary bypass acute kidney injury. BMC Genomics 15:916 (2014). PubMed: 25331815