Anti-SLC16A3/MCT 4 antibody (ab234728)
Key features and details
- Rabbit polyclonal to SLC16A3/MCT 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SLC16A3/MCT 4 antibody
See all SLC16A3/MCT 4 primary antibodies -
Description
Rabbit polyclonal to SLC16A3/MCT 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SLC16A3/MCT 4 aa 400 to the C-terminus.
Database link: O15427 -
Positive control
- WB: HeLa and MCF-7. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
ICC/IF
1/50 - 1/200. |
Target
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Function
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. -
Tissue specificity
Highly expressed in skeletal muscle. -
Sequence similarities
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9123 Human
- Omim: 603877 Human
- SwissProt: O15427 Human
- Unigene: 500761 Human
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Form
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains. -
Alternative names
- MCT 4 antibody
- MCT3 antibody
- MCT4 antibody
see all
Images
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All lanes : Anti-SLC16A3/MCT 4 antibody (ab234728) at 1/2500 dilution
Lane 1 : MCF-7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/5000 dilution
Predicted band size: 49 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC16A3/MCT 4 using ab234728 at a dilution of 1/100 in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab234728 has been referenced in 3 publications.
- Deguchi H et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy. Sci Rep 12:6263 (2022). PubMed: 35428816
- Shrestha P et al. Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes. Anal Cell Pathol (Amst) 2022:6718566 (2022). PubMed: 36340268
- Mou Z et al. Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. Front Genet 11:566918 (2020). PubMed: 33424916