Key features and details
- Rabbit polyclonal to SLC16A3/MCT 4 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC16A3/MCT 4 antibody - C-terminal
See all SLC16A3/MCT 4 primary antibodies
DescriptionRabbit polyclonal to SLC16A3/MCT 4 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla
- HeLa, Jurkat, 293T whole cell lysate
Protein previously labeled as SLC16A3.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 49 kDa.|
FunctionProton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Tissue specificityHighly expressed in skeletal muscle.
Sequence similaritiesBelongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Cellular localizationCell membrane.
- Information by UniProt
FormLactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains.
- MCT 4 antibody
- MCT3 antibody
- MCT4 antibody
All lanes : Anti-SLC16A3/MCT 4 antibody - C-terminal (ab190497) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutes
Immunoprecipitation analysis of HeLa cell lysate (1 mg per IP reaction; 20% of IP loaded) labeling SLC16A3/MCT 4 using ab190497 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated SLC16A3, ab190497 was used at 0.4 μg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab190497 has not yet been referenced specifically in any publications.