Key features and details
- Rabbit polyclonal to SLC19A3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC19A3 antibody
See all SLC19A3 primary antibodies
DescriptionRabbit polyclonal to SLC19A3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: SH-SY5Y, HepG2, HEK-293 and HeLa whole cell lysate (ab150035). ICC/IF: HepG2 cells.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237630 in the following tested applications.
|WB||1/500 - 1/5000. Predicted molecular weight: 56 kDa.|
|ICC/IF||1/50 - 1/200.|
RelevanceSLC19A3 is a member of the reduced folate family of micronutrient transporter genes. It is a ubiquitously expressed transmembrane thiamine transporter that lacks folate transport activity yet mediates high affinity thiamine uptake propably via a proton anti-port mechanism. Mutations in this gene cause biotin-responsive basal ganglia disease (BBGD); a recessive disorder manifested in childhood that progresses to chronic encephalopathy, dystonia, quadriparesis, and death if untreated. Patients with BBGD have bilateral necrosis in the head of the caudate nucleus and in the putamen. Mutations in this gene also cause a Wernicke's-like encephalopathy.
Cellular localizationPlasma membrane
- Solute carrier family 19 member 3 antibody
- Thiamine transporter 2 antibody
- thTr 2 antibody
- THTR2 antibody
All lanes : Anti-SLC19A3 antibody (ab237630) at 1/500 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC19A3 (green) using ab237630 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237630 has not yet been referenced specifically in any publications.