Key features and details
- Rabbit polyclonal to SLC1A5/ASCT2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC1A5/ASCT2 antibody
See all SLC1A5/ASCT2 primary antibodies
DescriptionRabbit polyclonal to SLC1A5/ASCT2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
This product was previously labelled as SLC1A5
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab187692 was affinity purified using an epitope specific to SLC1A5/ASCT2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab187692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
FunctionSodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
Tissue specificityPlacenta, lung, skeletal muscle, kidney, pancreas, and intestine.
Sequence similaritiesBelongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.
Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- ASCT2 antibody
- AAAT antibody
- AAAT_HUMAN antibody
All lanes : Anti-SLC1A5/ASCT2 antibody (ab187692) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 3 minutes
Detection of SLC1A5/ASCT2 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab187692 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab187692 has been referenced in 2 publications.
- Huang X et al. The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. Autophagy 15:1258-1279 (2019). PubMed: 30786811
- Sen N et al. EWS-FLI1 reprograms the metabolism of Ewing sarcoma cells via positive regulation of glutamine import and serine-glycine biosynthesis. Mol Carcinog 57:1342-1357 (2018). PubMed: 29873416