Overview

  • Product name

    Anti-SLC1A5/ASCT2 antibody [CAL33]
    See all SLC1A5/ASCT2 primary antibodies
  • Description

    Rabbit monoclonal [CAL33] to SLC1A5/ASCT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human colon carcinoma and lung carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. ICC/IF: Jurkat and HeLa cells. Flow Cyt: Jurkat and HeLa cells. IP: Jurkat and HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SLC1A5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab237704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 56 kDa.
ICC/IF 1/50.
Flow Cyt 1/40.
IP 1/30.

Target

  • Function

    Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
  • Tissue specificity

    Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.
  • Sequence similarities

    Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.
  • Cellular localization

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASCT2 antibody
    • AAAT antibody
    • AAAT_HUMAN antibody
    • ATB(0) antibody
    • ATBO antibody
    • Baboon M7 virus receptor antibody
    • FLJ31068 antibody
    • M7V1 antibody
    • M7VS1 antibody
    • Neutral amino acid transporter B(0) antibody
    • R16 antibody
    • RD114/simian type D retrovirus receptor antibody
    • RDR antibody
    • RDRC antibody
    • SLC1A5 antibody
    • Sodium dependent neutral amino acid transporter type 2 antibody
    • Sodium-dependent neutral amino acid transporter type 2 antibody
    • Solute carrier family 1 (neutral amino acid transporter), member 5 antibody
    • Solute carrier family 1 member 5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human colon carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling SLC1A5/ASCT2 with ab237704 at 1/1600 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human lung carcinoma is observed. The section was incubated with ab237704 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SLC1A5/ASCT2 using ab237704 at 0.5 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-SLC1A5/ASCT2 antibody [CAL33] (ab237704) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 100% methanol fixed Jurkat (human T cell leukemia T lymphocyte) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SLC1A5/ASCT2 with ab237704 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HeLa cells. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cell line labeling SLC1A5/ASCT2 with ab237704 at 1/40 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

  • SLC1A5/ASCT2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab237704 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237704 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Jurkat whole cell lysate 10 µg (Input).
    Lane 2: ab237704 IP in Jurkat whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237704 in Jurkat whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    ASCT2 (SLC1A5) can form dimers or trimers based on literature. (PMID: 23756778).

References

ab237704 has not yet been referenced specifically in any publications.

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