Key features and details
- Rabbit polyclonal to SLC20A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC20A1 antibody
DescriptionRabbit polyclonal to SLC20A1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cat, Orangutan
Recombinant fragment corresponding to Human SLC20A1 aa 263-458.
KCSPSESPLMEKKNSLKEDHEETKLSVGDIENKHPVSEVGPATVPLQAVV EERTVSFKLGDLEEAPERERLPSVDLKEETSIDSTVNGAVQLPNGNLVQF SQAVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPL RRNNSYTSYTMAICGMPLDSFRAKEGEQKGEEMEKLTWPNADSKKR
Database link: Q8WUM9
- WB: HeLa, HepG2 and MCF7 whole cell lysate. IHC-P: Human ovarian cancer and liver tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 74 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionSodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
DomainRegion A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.
- Information by UniProt
- DKFZp686J2397 antibody
- FLJ41426 antibody
- Gibbon ape leukemia virus receptor 1 antibody
All lanes : Anti-SLC20A1 antibody (ab237527) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SLC20A1 (green) using ab237527 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human ovarian cancer tissue stained for SLC20A1 with ab237527 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for SLC20A1 with ab237527 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237527 has been referenced in 1 publication.
- Li J et al. Impact of SLC20A1 on the Wnt/ß-catenin signaling pathway in somatotroph adenomas. Mol Med Rep 20:3276-3284 (2019). PubMed: 31432167