Overview

  • Product name
    Anti-SLC20A1 antibody [EPR11427(2)]
    See all SLC20A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11427(2)] to SLC20A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLC20A1 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8WUM9

  • Positive control
    • Fetal liver, HeLa, 293T and Jurkat lysates; Human ovarian carcinoma and uterus tissues; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
    • Domain
      Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686J2397 antibody
      • FLJ41426 antibody
      • Gibbon ape leukemia virus receptor 1 antibody
      • GLVR 1 antibody
      • GLVR-1 antibody
      • GLVR1 antibody
      • Leukemia virus receptor 1 homolog antibody
      • Phosphate transporter 1 antibody
      • PIT 1 antibody
      • PiT-1 antibody
      • PIT1 antibody
      • S20A1_HUMAN antibody
      • Slc20a1 antibody
      • Sodium dependent phosphate transporter 1 antibody
      • Sodium-dependent phosphate transporter 1 antibody
      • Solute carrier family 20 (phosphate transporter) member 1 antibody
      • Solute carrier family 20 member 1 antibody
      see all

    Images

    • ab177147 staining SLC20A1 antibody in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1:PBS only.

    • Immunofluorescent analysis of HeLa cells labeling SLC20A1 with ab177147 at 1/50 dilution.

    • All lanes : Anti-SLC20A1 antibody [EPR11427(2)] (ab177147) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa lysate
      Lane 3 : 293T lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 74 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling SLC20A1 with ab177147 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling SLC20A1 with ab177147 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SLC20A1 with ab177147 at 1/50 dilution.

    References

    ab177147 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (aortic valve interstitial cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    aortic valve interstitial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Oct 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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