Product nameAnti-SLC20A1 antibody [EPR11427(2)]
See all SLC20A1 primary antibodies
DescriptionRabbit monoclonal [EPR11427(2)] to SLC20A1
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SLC20A1 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WUM9
- Fetal liver, HeLa, 293T and Jurkat lysates; Human ovarian carcinoma and uterus tissues; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 74 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
DomainRegion A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.
- Information by UniProt
- DKFZp686J2397 antibody
- FLJ41426 antibody
- Gibbon ape leukemia virus receptor 1 antibody
ab177147 staining SLC20A1 antibody in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
Immunofluorescent analysis of HeLa cells labeling SLC20A1 with ab177147 at 1/50 dilution.
All lanes : Anti-SLC20A1 antibody [EPR11427(2)] (ab177147) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Flow cytometric analysis of permeabilized HeLa cells labeling SLC20A1 with ab177147 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling SLC20A1 with ab177147 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SLC20A1 with ab177147 at 1/50 dilution.
ab177147 has not yet been referenced specifically in any publications.