Product nameAnti-SLC22A1/OCT1 antibody [EPR3668(N)]
See all SLC22A1/OCT1 primary antibodies
DescriptionRabbit monoclonal [EPR3668(N)] to SLC22A1/OCT1
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human SLC22A1/OCT1 aa 100-200. The exact sequence is proprietary.
Database link: O15245
- Jurkat, SW480, HepG2 and Caco-2 cell lysate. HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 61 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranslocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.
Tissue specificityWidely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines.
Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
Cellular localizationBasolateral cell membrane.
- Information by UniProt
- hOCT1 antibody
- OCT1 antibody
- oct1_cds antibody
All lanes : Anti-SLC22A1/OCT1 antibody [EPR3668(N)] (ab181022) at 1/10000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Flow Cytometry analysis of HepG2 cells labeling SLC22A1/OCT1 using ab181022 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
This product has been referenced in:
- Thakur S et al. Metformin Targets Mitochondrial Glycerophosphate Dehydrogenase to Control Rate of Oxidative Phosphorylation and Growth of Thyroid Cancer In Vitro and In Vivo. Clin Cancer Res N/A:N/A (2018). Read more (PubMed: 29691295) »