Overview

  • Product name

    Anti-SLC22A2 antibody [EPR11248]
    See all SLC22A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11248] to SLC22A2
  • Host species

    Rabbit
  • Specificity

    Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLC22A2 aa 100-200. The exact sequence is proprietary.
    Database link: O15244

  • Positive control

    • Human fetal kidney lysate, 293T, HepG2 and A431 lysates, Human kidney tissue and Human prostate tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11248
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Relevance

      SLC22A2 (solute carrier family 22 (organic cation transporter), member 2) is an organic cation transporter. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A2 is found primarily in the kidney, where it may mediate the first step in cation reabsorption. It plays important roles in the renal tubular secretion of cationic drugs.
    • Cellular localization

      Plasma membrane
    • Database links

    • Alternative names

      • MGC32628 antibody
      • OCT 2 antibody
      • OCT2 antibody
      • Organic cation transporter 2 antibody
      • Organic cation transporter antibody
      • RP11-317M22.2 antibody
      • Solute carrier family 22 (organic cation transporter) member 2 antibody
      • Solute carrier family 22 member 2 antibody
      see all

    Images

    • ab170871 showing +ve staining in Human uterus tissue.

    • ab170871 showing +ve staining in Human normal stomach tissue.

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SLC22A2 using ab170871 at a 1/100 dilution. 

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC22A2 using ab170871 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab170871 showing -ve staining in Human normal liver tissue.
    • All lanes : Anti-SLC22A2 antibody [EPR11248] (ab170871) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : 293T lysate
      Lane 3 : HepG2 lysate
      Lane 4 : A431 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa

    References

    This product has been referenced in:

    • Wang S  et al. The flavonoid-rich fraction from rhizomes of Smilax glabra Roxb. ameliorates renal oxidative stress and inflammation in uric acid nephropathy rats through promoting uric acid excretion. Biomed Pharmacother 111:162-168 (2019). Read more (PubMed: 30579255) »
    • Sekhar GN  et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS One 12:e0173474 (2017). Read more (PubMed: 28362799) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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