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Mouse monoclonal [mAbcam75402] to SLC22A3
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SLC22A3.
(Peptide available as
This antibody gave a positive signal in Human Prostate Tissue Lysate.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
firstname.lastname@example.org or you can find further information here.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 10
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use a concentration of 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
Belongs to the major facilitator superfamily. Organic cation transporter family.
Information by UniProt
EMT organic cation transporter 3 antibody
Western blot - Anti-SLC22A3 antibody [mAbcam75402] (ab75402)
Anti-SLC22A3 antibody [mAbcam75402] (ab75402) at 5 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein (
) at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Flow Cytometry - Anti-SLC22A3 antibody [mAbcam75402] (ab75402)
Overlay histogram showing JEG3 cells stained with ab75402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75402, 1µg/1x10
cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (
cells) used under the same conditions. Acquisition of >5,000 events was performed.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"