Recombinant
RabMAb

Recombinant Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)

Overview

  • Product name

    Anti-SLC22A3/OCT3 antibody [EPR6630]
    See all SLC22A3/OCT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6630] to SLC22A3/OCT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC22A3/OCT3 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as SLC22A3 / EMT

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
    • Tissue specificity

      Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
    • Sequence similarities

      Belongs to the major facilitator superfamily. Organic cation transporter family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • EMT antibody
      • EMT organic cation transporter 3 antibody
      • EMTH antibody
      • Extraneuronal monoamine transporter antibody
      • OCT 3 antibody
      • OCT3 antibody
      • Orct 3 antibody
      • Orct3 antibody
      • Organic cation transporter 3 antibody
      • S22A3_HUMAN antibody
      • SLC22 A3 antibody
      • SLC22A 3 antibody
      • Slc22a3 antibody
      • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
      • Solute carrier family 22 (organic cation transporter) member 3 antibody
      • Solute carrier family 22 member 3 antibody
      see all

    Images

    • All lanes : Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human muscle lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 61 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab124826, at 1/100 dilution, staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Guttmann S  et al. Organic cation transporter 3 mediates cisplatin and copper cross-resistance in hepatoma cells. Oncotarget 9:743-754 (2018). Read more (PubMed: 29416650) »
    • Irannejad R  et al. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol 13:799-806 (2017). Read more (PubMed: 28553949) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.
    For WB 1-3%BSA in TBST and for IHC 1-3%BSA in PBST would be good for diluting the antibody.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for contacting us.
    The dilutions should always be optimized by end user. We can recommend a starting dilution of 1/500 for ab135721 and 1/50,000 for ab124826.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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