Product nameAnti-SLC22A3/OCT3 antibody [EPR6630]
See all SLC22A3/OCT3 primary antibodies
DescriptionRabbit monoclonal [EPR6630] to SLC22A3/OCT3
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human SLC22A3/OCT3 aa 300-400. The exact sequence is proprietary.
- Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as SLC22A3 / EMT
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.75 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
Tissue specificityExpressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
- Information by UniProt
- EMT antibody
- EMT organic cation transporter 3 antibody
- EMTH antibody
All lanes : Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human muscle lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab124826, at 1/100 dilution, staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Guttmann S et al. Organic cation transporter 3 mediates cisplatin and copper cross-resistance in hepatoma cells. Oncotarget 9:743-754 (2018). Read more (PubMed: 29416650) »
- Irannejad R et al. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol 13:799-806 (2017). Read more (PubMed: 28553949) »