Overview

  • Product name

    Anti-SLC22A3/OCT3 antibody [mAbcam75402]
    See all SLC22A3/OCT3 primary antibodies
  • Description

    Mouse monoclonal [mAbcam75402] to SLC22A3/OCT3
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SLC22A3/OCT3 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107919)

  • Positive control

    • This antibody gave a positive signal in Human Prostate Tissue Lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as SLC22A3 / EMT

     

Properties

Applications

Our Abpromise guarantee covers the use of ab75402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • Function

    Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
  • Tissue specificity

    Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EMT antibody
    • EMT organic cation transporter 3 antibody
    • EMTH antibody
    • Extraneuronal monoamine transporter antibody
    • OCT 3 antibody
    • OCT3 antibody
    • Orct 3 antibody
    • Orct3 antibody
    • Organic cation transporter 3 antibody
    • S22A3_HUMAN antibody
    • SLC22 A3 antibody
    • SLC22A 3 antibody
    • Slc22a3 antibody
    • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
    • Solute carrier family 22 (organic cation transporter) member 3 antibody
    • Solute carrier family 22 member 3 antibody
    see all

Images

  • Anti-SLC22A3/OCT3 antibody [mAbcam75402] (ab75402) at 5 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein (ab30307) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 16 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Overlay histogram showing JEG3 cells stained with ab75402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75402, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

ab75402 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75402.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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