Key features and details
- Mouse monoclonal [mAbcam75402] to SLC22A3/OCT3
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-SLC22A3/OCT3 antibody [mAbcam75402]
See all SLC22A3/OCT3 primary antibodies
DescriptionMouse monoclonal [mAbcam75402] to SLC22A3/OCT3
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human SLC22A3/OCT3 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Prostate Tissue Lysate.
This antibody clone is manufactured by Abcam.
This product was previously labelled as SLC22A3 / EMT
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab75402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
FunctionMediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
Tissue specificityExpressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
- Information by UniProt
- EMT antibody
- EMT organic cation transporter 3 antibody
- EMTH antibody
Anti-SLC22A3/OCT3 antibody [mAbcam75402] (ab75402) at 5 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein (ab30307) at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Overlay histogram showing JEG3 cells stained with ab75402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75402, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab75402 has not yet been referenced specifically in any publications.