Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC22A6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a sequence at the C terminal of Human SLC22A6.

  • Positive control

    • Rat kidney tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • Tissue specificity

    Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • Domain

    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.
  • Post-translational
    modifications

    Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ55736 antibody
    • hOAT1 antibody
    • hPAHT antibody
    • hROAT1 antibody
    • MGC45260 antibody
    • OAT1 antibody
    • Organic anion transporter 1 antibody
    • OTTHUMP00000236796 antibody
    • OTTHUMP00000236797 antibody
    • OTTHUMP00000236798 antibody
    • OTTHUMP00000236799 antibody
    • PAH transporter antibody
    • PAHT antibody
    • Para aminohippurate transporter antibody
    • Renal organic anion transporter 1 antibody
    • ROAT1 antibody
    • S22A6_HUMAN antibody
    • SLC22A6 antibody
    • Solute carrier family 22 (organic anion transporter) member 6 antibody
    • Solute carrier family 22 member 6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Rat kidney tissue labelling SLC22A6 with ab131087 at 0.5 µg/ml.

References

This product has been referenced in:

  • Zhou Y  et al. Adefovir accumulation and nephrotoxicity in renal interstitium: Role of organic anion transporters of kidney. Life Sci 224:41-50 (2019). Read more (PubMed: 30902543) »
  • Wang S  et al. The flavonoid-rich fraction from rhizomes of Smilax glabra Roxb. ameliorates renal oxidative stress and inflammation in uric acid nephropathy rats through promoting uric acid excretion. Biomed Pharmacother 111:162-168 (2019). Read more (PubMed: 30579255) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidneys)
Permeabilization
No
Specification
Kidneys
Fixative
NA

Lukas Wijaya

Verified customer

Submitted Jun 25 2018

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