Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC22A6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of Human SLC22A6.

  • Positive control

    • Mouse liver cell extract; Human brain tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • Tissue specificity

    Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • Domain

    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.
  • Post-translational
    modifications

    Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ55736 antibody
    • hOAT1 antibody
    • hPAHT antibody
    • hROAT1 antibody
    • MGC45260 antibody
    • OAT1 antibody
    • Organic anion transporter 1 antibody
    • OTTHUMP00000236796 antibody
    • OTTHUMP00000236797 antibody
    • OTTHUMP00000236798 antibody
    • OTTHUMP00000236799 antibody
    • PAH transporter antibody
    • PAHT antibody
    • Para aminohippurate transporter antibody
    • Renal organic anion transporter 1 antibody
    • ROAT1 antibody
    • S22A6_HUMAN antibody
    • SLC22A6 antibody
    • Solute carrier family 22 (organic anion transporter) member 6 antibody
    • Solute carrier family 22 member 6 antibody
    see all

Images

  • Anti-SLC22A6 antibody (ab135924) at 1/500 dilution + Mouse liver cell extract at 30 µg

    Predicted band size: 62 kDa

  • ab135924 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135924 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SLC22A6 with ab135924 at 1/50 dilution.

References

This product has been referenced in:

  • Yong T  et al. Cordycepin, a Characteristic Bioactive Constituent inCordyceps militaris, Ameliorates Hyperuricemia through URAT1 in Hyperuricemic Mice. Front Microbiol 9:58 (2018). WB ; Mouse . Read more (PubMed: 29422889) »
  • Yong T  et al. Hypouricemic Effects of Extracts From Agrocybe aegerita on Hyperuricemia Mice and Virtual Prediction of Bioactives by Molecular Docking. Front Pharmacol 9:498 (2018). Read more (PubMed: 29867500) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, proximal tubule)
Specification
kidney, proximal tubule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted Apr 29 2019

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