Key features and details
- Rabbit polyclonal to SLC22A7/OAT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC22A7/OAT2 antibody
See all SLC22A7/OAT2 primary antibodies
DescriptionRabbit polyclonal to SLC22A7/OAT2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Pig
- Jurkat cell membrane lysate; Human muscle tissue
This product was previously labelled as SLC22A7
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionMediates sodium-independent multispecific organic anion transport. Transport of prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate.
Tissue specificityExpressed in liver and kidney.
Sequence similaritiesBelongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
Cellular localizationBasolateral cell membrane. Apical side of the renal tubule.
- Information by UniProt
- hOAT2 antibody
- liver specific transporter antibody
- NLT antibody
All lanes : Anti-SLC22A7/OAT2 antibody (ab58683) at 5 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Jurkat cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/5000 dilution
Observed band size: 58 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SLC22A7/OAT2 with ab58683 at 8µg/ml. Arrows indicate positive labelled skeletal muscle cells. Magnification: X400.
All lanes : Anti-SLC22A7/OAT2 antibody (ab58683) at 2.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml
Lysates/proteins at 25 µg per lane.
ab58683 has not yet been referenced specifically in any publications.