Key features and details
- Rabbit polyclonal to SLC24A1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC24A1 antibody
See all SLC24A1 primary antibodies
DescriptionRabbit polyclonal to SLC24A1
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human retina tissue. ICC/IF: A431 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionCritical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+).
Tissue specificityExpressed in the retina, particularly in the inner segment, outer and inner nuclear layers, and ganglion cell layer.
Involvement in diseaseDefects in SLC24A1 are the cause of congenital stationary night blindness type 1D (CSNB1D) [MIM:613830]. An autosomal recessive form of congenital stationary night blindness a non-progressive retinal disorder characterized by impaired night vision. CSNB1D is characterized by a Riggs type of electroretinogram (proportionally reduced a- and b-waves). Patients have visual acuity within the normal range and no symptoms of myopia and/or nystagmus.
Sequence similaritiesBelongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.
modificationsThe uncleaved signal sequence is required for efficient membrane targeting and proper membrane integration.
- Information by UniProt
- CSNB1D antibody
- HsT17412 antibody
- KIAA0702 antibody
PFA fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling SLC24A1 using ab243573 at 4 µg/ml (green) in ICC/IF. Nuclear probe is visualized in blue.
Formalin-fixed, paraffin-embedded human retina tissue stained for SLC24A1 with ab243573 at a 1/1000 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243573 has not yet been referenced specifically in any publications.