Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC24A6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: SIGDAFSDFT LARQGYPRMA FSACFGGIIF NILVGVGLGC LLQISRSHTE , corresponding to a region within internal sequence amino acids 468 - 517 of Human SLC24A6 (NP_079235)

  • Positive control

    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Transports Ca(2+) in exchange for either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange. In contrast to other members of the family its function is independent of K(+).
  • Sequence similarities

    Belongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ22233 antibody
    • Na(+)/K(+)/Ca(2+) exchange protein 6 antibody
    • Na(+)/K(+)/Ca(2+)-exchange protein 6 antibody
    • NCKX6 antibody
    • NCKX6_HUMAN antibody
    • NCLX antibody
    • Slc24a6 antibody
    • Sodium/potassium/calcium exchanger antibody
    • Sodium/potassium/calcium exchanger 6 antibody
    • Solute carrier family 24 (sodium/potassium/calcium exchanger) member 6 antibody
    • Solute carrier family 24 member 6 antibody
    see all

Images

  • Anti-SLC24A6 antibody (ab83551) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa
    Additional bands at: 35 kDa, 95 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12%

References

This product has been referenced in:

  • Xie A  et al. Mitochondrial Ca2+ Influx Contributes to Arrhythmic Risk in Nonischemic Cardiomyopathy. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29627768) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Guinea pig Cell lysate - other (heart mitochondria)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
heart mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ting

Verified customer

Submitted Aug 11 2015

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