Overview

  • Product name
  • Description
    Rabbit polyclonal to Slc25a1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Slc25a1 aa 149-178 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_005975.1.
    Database link: P53007

  • Positive control
    • Jurkat cell lysate and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab174924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function
    Involved in citrate-H(+)/malate exchange. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic pathway.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Citrate transport protein antibody
    • CTP antibody
    • mitochondrial antibody
    • SLC20A3 antibody
    • Slc25a1 antibody
    • solute carrier family 20 (mitochondrial citrate transporter), member 3 antibody
    • solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1 antibody
    • Solute carrier family 25 member 1 antibody
    • Tricarboxylate carrier protein antibody
    • Tricarboxylate transport protein antibody
    • tricarboxylate transport protein, mitochondrial antibody
    • TXTP_HUMAN antibody
    see all

Images

  • Anti-Slc25a1 antibody (ab174924) at 1/1000 dilution + Jurkat cell lysate at 35 µg

    Predicted band size: 34 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue labeling Slc25a1 using ab174924 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using ab174924 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Fernandez HR  et al. The mitochondrial citrate carrier, SLC25A1, drives stemness and therapy resistance in non-small cell lung cancer. Cell Death Differ 25:1239-1258 (2018). Read more (PubMed: 29651165) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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