Product nameAnti-SLC25A12 antibody
See all SLC25A12 primary antibodies
DescriptionRabbit polyclonal to SLC25A12
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Cat, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Mouse SLC25A12 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q8BH59
- This antibody gave a positive signal in the following Mouse tissue lysates: Retina; E14 Embryonic Brain; Heart; Skeletal Muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
FunctionCalcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.
Tissue specificityHigh levels in heart and skeletal muscle, low in brain and very low in kidney.
Involvement in diseaseDefects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AGC1 antibody
- Araceli hiperlarga antibody
- ARALAR antibody
All lanes : Anti-SLC25A12 antibody (ab138529) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 12,19,74 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa (possible non-specific binding)
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab138529 has not yet been referenced specifically in any publications.