Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC25A12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Cat, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse SLC25A12 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q8BH59

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissue lysates: Retina; E14 Embryonic Brain; Heart; Skeletal Muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab138529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

Target

  • Function

    Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.
  • Tissue specificity

    High levels in heart and skeletal muscle, low in brain and very low in kidney.
  • Involvement in disease

    Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 4 EF-hand domains.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGC1 antibody
    • Araceli hiperlarga antibody
    • ARALAR antibody
    • ARALAR1 antibody
    • Calcium binding mitochondrial carrier superfamily member antibody
    • Calcium binding mitochondrial carrier superfamily member Aralar1 antibody
    • Calcium-binding mitochondrial carrier protein Aralar1 antibody
    • CMC1_HUMAN antibody
    • Mitochondrial aspartate glutamate carrier 1 antibody
    • SLC25A12 antibody
    • Solute carrier family 25 (aspartate/glutamate carrier) member 12 antibody
    • Solute carrier family 25 (mitochondrial carrier Aralar) member 12 antibody
    • Solute carrier family 25 member 12 antibody
    • solute carrier family 25, member 12 antibody
    see all

Images

  • All lanes : Anti-SLC25A12 antibody (ab138529) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 12,19,74 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa (possible non-specific binding)


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab138529 has not yet been referenced specifically in any publications.

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