Anti-SLC25A12 antibody (ab234975)
Key features and details
- Rabbit polyclonal to SLC25A12
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC25A12 antibody
See all SLC25A12 primary antibodies -
Description
Rabbit polyclonal to SLC25A12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SLC25A12 aa 202-317.
Database link: O75746 -
Positive control
- WB: HEK-293 whole cell lysate. Mouse and rat heart and kidney tissue lysates. IHC-P: Human pancreatic cancer and brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 74 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 74 kDa. |
Target
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Function
Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle. -
Tissue specificity
High levels in heart and skeletal muscle, low in brain and very low in kidney. -
Involvement in disease
Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 8604 Human
- Entrez Gene: 78830 Mouse
- Entrez Gene: 362145 Rat
- Omim: 603667 Human
- SwissProt: O75746 Human
- SwissProt: Q8BH59 Mouse
- Unigene: 470608 Human
- Unigene: 30928 Mouse
see all -
Alternative names
- AGC1 antibody
- Araceli hiperlarga antibody
- ARALAR antibody
see all
Images
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All lanes : Anti-SLC25A12 antibody (ab234975) at 1/500 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa -
Paraffin-embedded human brain tissue stained for SLC25A12 with ab234975 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreatic cancer tissue stained for SLC25A12 with ab234975 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234975 has not yet been referenced specifically in any publications.