Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

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Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
  • Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16294] to SLC25A12 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free
    See all SLC25A12 primary antibodies

  • Description

    Rabbit monoclonal [EPR16294] to SLC25A12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat whole cell lysate.

  • General notes

    ab246329 is the carrier-free version of ab200201.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab246329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.

  • Tissue specificity

    High levels in heart and skeletal muscle, low in brain and very low in kidney.

  • Involvement in disease

    Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.

  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 4 EF-hand domains.
    Contains 3 Solcar repeats.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AGC1 antibody
    • Araceli hiperlarga antibody
    • ARALAR antibody
    • ARALAR1 antibody
    • Calcium binding mitochondrial carrier superfamily member antibody
    • Calcium binding mitochondrial carrier superfamily member Aralar1 antibody
    • Calcium-binding mitochondrial carrier protein Aralar1 antibody
    • CMC1_HUMAN antibody
    • Mitochondrial aspartate glutamate carrier 1 antibody
    • SLC25A12 antibody
    • Solute carrier family 25 (aspartate/glutamate carrier) member 12 antibody
    • Solute carrier family 25 (mitochondrial carrier Aralar) member 12 antibody
    • Solute carrier family 25 member 12 antibody
    • solute carrier family 25, member 12 antibody
    see all

Images

  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
    Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input).

    Lane 2: ab200201 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time (Panel A and Panel B): 30 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).

  • Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
    Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

    SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input).

    Lane 2: ab200201 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).

  • Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
    Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab246329? Please let us know so that we can cite the reference in this datasheet.

ab246329 has not yet been referenced specifically in any publications.

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