Recombinant Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free
See all SLC25A12 primary antibodies -
Description
Rabbit monoclonal [EPR16294] to SLC25A12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SLC25A12 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: O75746 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
Ab246329 is the carrier-free version of ab200201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab246329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle. -
Tissue specificity
High levels in heart and skeletal muscle, low in brain and very low in kidney. -
Involvement in disease
Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 8604 Human
- Entrez Gene: 78830 Mouse
- Entrez Gene: 362145 Rat
- Omim: 603667 Human
- SwissProt: O75746 Human
- SwissProt: Q8BH59 Mouse
- Unigene: 470608 Human
- Unigene: 30928 Mouse
see all -
Alternative names
- AGC1 antibody
- Araceli hiperlarga antibody
- ARALAR antibody
see all
Images
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![Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)](https://www.abcam.com/ps/products/246/ab246329/Images/ab246329-2-ab200201245090ab200201IPb.jpg)
Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time (Panel A and Panel B): 30 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).
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![Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)](https://www.abcam.com/ps/products/246/ab246329/Images/ab246329-1-ab200201245091ab200201IPa.jpg)
Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 second.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).
Datasheets and documents
References
ab246329 has not yet been referenced specifically in any publications.
