Recombinant Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
![Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)](https://www.abcam.com/ps/products/246/ab246329/Images/ab246329-2-ab200201245090ab200201IPb.jpg "Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16294] to SLC25A12 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free
See all SLC25A12 primary antibodies -
Description
Rabbit monoclonal [EPR16294] to SLC25A12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SLC25A12 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: O75746 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
Ab246329 is the carrier-free version of ab200201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab246329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16294 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab246329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle. -
Tissue specificity
High levels in heart and skeletal muscle, low in brain and very low in kidney. -
Involvement in disease
Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8604 Human
- Entrez Gene: 78830 Mouse
- Entrez Gene: 362145 Rat
- Omim: 603667 Human
- SwissProt: O75746 Human
- SwissProt: Q8BH59 Mouse
- Unigene: 470608 Human
- Unigene: 30928 Mouse
see all -
Alternative names
- AGC1 antibody
- Araceli hiperlarga antibody
- ARALAR antibody
see all
Images
-
Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time (Panel A and Panel B): 30 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).
-
![Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)](https://www.abcam.com/ps/products/246/ab246329/Images/ab246329-1-ab200201245091ab200201IPa.jpg)
Immunoprecipitation - Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 second.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200201).
-
![Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)](https://www.abcam.com/ps/products/246/ab246329/Images/ab246329-3-benefits-of-recombinant-antibodies.png)
Anti-SLC25A12 antibody [EPR16294] - BSA and Azide free (ab246329)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab246329 has not yet been referenced specifically in any publications.
