Product nameAnti-SLC25A13 antibody [EPR9968(B)]
See all SLC25A13 primary antibodies
DescriptionRabbit monoclonal [EPR9968(B)] to SLC25A13
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human SLC25A13 (Q9UJS0).
- Fetal liver, HepG2, and SH-SY5Y cell lysates, Human liver tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCatalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.
Tissue specificityHigh levels in liver and low levels in kidney, pancreas, placenta, heart and brain.
Involvement in diseaseDefects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: SLC25A13 knockout HAP1 cell lysate (40 µg)
Lane 3: (40 µg)
Lane 4: (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab156010 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab156010 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. Ab156010 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-SLC25A13 antibody [EPR9968(B)] (ab156010) at 1/1000 dilution
Lane 1 : fetal liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling SLC25A13 with ab156010 antibody at 1/50.
ab156010 has not yet been referenced specifically in any publications.