Anti-SLC25A13/Citrin antibody (ab96303)
Key features and details
- Rabbit polyclonal to SLC25A13/Citrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SLC25A13/Citrin antibody
See all SLC25A13/Citrin primary antibodies -
Description
Rabbit polyclonal to SLC25A13/Citrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human SLC25A13/Citrin aa 248-505.
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Positive control
- IHC-P: Mouse kidney tissue;N87 xenograft tissue. ICC/IF: HeLa cells. WB: Mouse liver lysate; rat liver lysate; HepG2, HEK-293T, A431 and HeLa whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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| Notes |
|---|
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
Target
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Function
Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. -
Tissue specificity
High levels in liver and low levels in kidney, pancreas, placenta, heart and brain. -
Involvement in disease
Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. -
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 615470 Cow
- Entrez Gene: 10165 Human
- Entrez Gene: 50799 Mouse
- Entrez Gene: 362322 Rat
- Omim: 603859 Human
- SwissProt: Q9UJS0 Human
- SwissProt: Q9QXX4 Mouse
- Unigene: 489190 Human
see all -
Alternative names
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SLC25A13/Citrin antibody (ab96303)
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SLC25A13/Citrin using ab96303 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Western blot - Anti-SLC25A13/Citrin antibody (ab96303)All lanes : Anti-SLC25A13/Citrin antibody (ab96303) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)Paraffin-embedded mouse kidney tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-SLC25A13/Citrin antibody (ab96303)Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + Mouse liver lysate at 50 µg
Predicted band size: 74 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)Paraffin-embedded N87 xenograft tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EWDTA-based (pH 8.0), 15 minutes.
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Western blot - Anti-SLC25A13/Citrin antibody (ab96303)Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 74 kDa -
Western blot - Anti-SLC25A13/Citrin antibody (ab96303)Anti-SLC25A13/Citrin antibody (ab96303) at 1/500 dilution + Rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96303 has not yet been referenced specifically in any publications.