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  1. Link

    slc25a13citrin-antibody-ab96303.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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Anti-SLC25A13/Citrin antibody (ab96303)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-SLC25A13/Citrin antibody (ab96303)
  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)
  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)
  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)

Key features and details

  • Rabbit polyclonal to SLC25A13/Citrin
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SLC25A13/Citrin antibody
    See all SLC25A13/Citrin primary antibodies
  • Description

    Rabbit polyclonal to SLC25A13/Citrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human SLC25A13/Citrin aa 248-505.

  • Positive control

    • IHC-P: Mouse kidney tissue;N87 xenograft tissue. ICC/IF: HeLa cells. WB: Mouse liver lysate; rat liver lysate; HepG2, HEK-293T, A431 and HeLa whole cell extracts.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Aspartate
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Other
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
WB (1)
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
Notes
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa.

Target

  • Function

    Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.
  • Tissue specificity

    High levels in liver and low levels in kidney, pancreas, placenta, heart and brain.
  • Involvement in disease

    Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
    Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 4 EF-hand domains.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession Q9UJS0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 615470 Cow
    • Entrez Gene: 10165 Human
    • Entrez Gene: 50799 Mouse
    • Entrez Gene: 362322 Rat
    • Omim: 603859 Human
    • SwissProt: Q9UJS0 Human
    • SwissProt: Q9QXX4 Mouse
    • Unigene: 489190 Human
    • Unigene: 24513 Mouse
    see all
  • Alternative names

    • AI785475 antibody
    • ARALAR2 antibody
    • Calcium binding mitochondrial carrier protein Aralar2 antibody
    • Calcium-binding mitochondrial carrier protein Aralar2 antibody
    • Citrin antibody
    • CMC2_HUMAN antibody
    • CTLN2 antibody
    • Ctrn antibody
    • Mitochondrial aspartate glutamate carrier 2 antibody
    • RGD1565889 antibody
    • Slc25a13 antibody
    • Solute carrier family 25 (citrin) member 13 antibody
    • Solute carrier family 25 member 13 (citrin) antibody
    • Solute carrier family 25 member 13 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC25A13/Citrin antibody (ab96303)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC25A13/Citrin antibody (ab96303)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SLC25A13/Citrin using ab96303 at 1/500 dilution (green)  in ICC/IF. 

    The nuclear counter stain is Hoechst 33342 (blue).

  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    All lanes : Anti-SLC25A13/Citrin antibody (ab96303) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)

    Paraffin-embedded mouse kidney tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.

    Antigen retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + Mouse liver lysate at 50 µg

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC25A13/Citrin antibody (ab96303)

    Paraffin-embedded N87 xenograft tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.

    Antigen retrieval: EWDTA-based (pH 8.0), 15 minutes.

  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 74 kDa

  • Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Western blot - Anti-SLC25A13/Citrin antibody (ab96303)
    Anti-SLC25A13/Citrin antibody (ab96303) at 1/500 dilution + Rat liver lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 74 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab96303? Please let us know so that we can cite the reference in this datasheet.

ab96303 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-SLC25A13/Citrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7, hepatocyte-derived carcinoma cell line)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Huh7, hepatocyte-derived carcinoma cell line
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Oct 14 2019

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