Product nameAnti-SLC25A13/Citrin antibody [EPR9969(B)]
See all SLC25A13/Citrin primary antibodies
DescriptionRabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human SLC25A13/Citrin (C terminal). The exact sequence is proprietary.
- Human fetal liver, HepG2 and SH-SY5Y cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as SLC25A13
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 70 kDa.|
FunctionCatalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.
Tissue specificityHigh levels in liver and low levels in kidney, pancreas, placenta, heart and brain.
Involvement in diseaseDefects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SLC25A13 knockout HAP1 cell lysate (20 µg)
Lane 3: SH-5YSY cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 cell lysates
Lane 3 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa
ab167166 has not yet been referenced specifically in any publications.