Overview

  • Product name

  • Description

    Rabbit polyclonal to SLC25A20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 215-264 (GGIAGIFNWA VAIPPDVLKS RFQTAPPGKY PNGFRDVLRE LIRDEGVTSL) of Human SLC25A20 (NP_000378).

  • Positive control

    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • Relevance

    SLC25A20 is one of several closely related mitochondrial membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. It mediates the transport of acylcarnitines into the mitochondrial matrix for their oxidation by the mitochondrial fatty acid oxidation pathway. Mutations in this gene are associated with carnitine acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
  • Cellular localization

    Mitochondrion inner membrane; Multipass membrane protein.
  • Database links

  • Alternative names

    • CAC antibody
    • CACT antibody
    • Carnitine/acylcarnitine translocase antibody
    • MCAT_HUMAN antibody
    • Mitochondrial carnitine/acylcarnitine carrier protein antibody
    • Solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 antibody
    • Solute carrier family 25 member 20 antibody
    see all

Images

  • Anti-SLC25A20 antibody (ab82678) at 1 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 33 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Gel concentration 12%

References

This product has been referenced in:

  • Soni MS  et al. Downregulation of carnitine acyl-carnitine translocase by miRNAs 132 and 212 amplifies glucose-stimulated insulin secretion. Diabetes 63:3805-14 (2014). WB ; Rat . Read more (PubMed: 24969106) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (A375)
Loading amount
20 µg
Specification
A375
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 21 2011

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