Key features and details
- Rabbit polyclonal to SLC25A20
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC25A20 antibody
See all SLC25A20 primary antibodies
DescriptionRabbit polyclonal to SLC25A20
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Jurkat cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab82678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
RelevanceSLC25A20 is one of several closely related mitochondrial membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. It mediates the transport of acylcarnitines into the mitochondrial matrix for their oxidation by the mitochondrial fatty acid oxidation pathway. Mutations in this gene are associated with carnitine acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
Cellular localizationMitochondrion inner membrane; Multipass membrane protein.
- CAC antibody
- CACT antibody
- Carnitine/acylcarnitine translocase antibody
Anti-SLC25A20 antibody (ab82678) at 1 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab82678 has been referenced in 1 publication.
- Soni MS et al. Downregulation of carnitine acyl-carnitine translocase by miRNAs 132 and 212 amplifies glucose-stimulated insulin secretion. Diabetes 63:3805-14 (2014). WB ; Rat . PubMed: 24969106