Key features and details
- Rabbit polyclonal to SLC25A25/APC3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SLC25A25/APC3 antibody
DescriptionRabbit polyclonal to SLC25A25/APC3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SLC25A25/APC3 aa 1-189.
Database link: Q6KCM7
- WB: Mouse liver and brain tissue lysates. IHC-P: Human prostate cancer. ICC/IF: U-251 MG cells.
This product was previously labelled as SLC25A25
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab237051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCalcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria.
Tissue specificityPresent in various cell lines (at protein level). Widely expressed. Expressed in fetal and adult liver, skeletal muscle, testis, ovary, hippocampus and caudate nucleus. Isoform 1 is present in all tissues tested. Isoform 2 expression is restricted to kidney and lung.
Sequence similaritiesBelongs to the mitochondrial carrier (TC 2.A.29) family.
Contains 3 EF-hand domains.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Calcium-binding mitochondrial carrier protein SCaMC-2 antibody
- KIAA1896 antibody
- MCSC antibody
All lanes : Anti-SLC25A25/APC3 antibody (ab237051) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 52 kDa
Paraffin-embedded human prostate cancer tissue stained for SLC25A25/APC3 using ab237051 at 1/100 dilution in immunohistochemical analysis.
U-251 MG (human brain glioma cell line) cells stained for SLC25A25/APC3 (green) using ab237051 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237051 has not yet been referenced specifically in any publications.