Key features and details
- Rabbit polyclonal to SLC25A28
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC25A28 antibody
See all SLC25A28 primary antibodies
DescriptionRabbit polyclonal to SLC25A28
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
- WB: MCF7 cell lysate. IHC-P: Human kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab80467 is purified by a peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab80467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceSLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells.
Cellular localizationMitochondrion inner membrane; Multi pass membrane protein. Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
- MFRN2 antibody
- Mitochondrial iron transporter 2 antibody
- Mitochondrial RNA splicing protein 3/4 antibody
Anti-SLC25A28 antibody (ab80467) at 1 µg/ml + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Western blot analysis of MCF7 cell lysate labeling SLC25A28 with ab80467.
ab80467 at 5 µg/ml staining SLC25A28 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab80467 has not yet been referenced specifically in any publications.