Key features and details
- Mouse polyclonal to SLC25A3/PHC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC25A3/PHC antibody
See all SLC25A3/PHC primary antibodies
DescriptionMouse polyclonal to SLC25A3/PHC
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human SLC25A3/PHC.
Database link: NP_002626.1
- Human pancreas tissue lysate, SLC25A3/PHC transfected 293T cell line lysate and HeLa cells.
This product was previously labelled as SLC25A3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab89117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionTransport of phosphate groups from the cytosol to the mitochondrial matrix. Phosphate is cotransported with H(+).
Involvement in diseaseDefects in SLC25A3 are a cause of mitochondrial phosphate carrier deficiency (MPCD) [MIM:610773]. MPCD is a fatal disorder of oxidative phosphorylation. Patients have lactic acidosis, hypertrophic cardiomyopathy and muscular hypotonia and die within the first year of life.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- mitochondrial antibody
- Mitochondrial phosphate carrier protein antibody
- MPCP_HUMAN antibody
Anti-SLC25A3/PHC antibody (ab89117) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 40 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
All lanes : Anti-SLC25A3/PHC antibody (ab89117) at 1 µg/ml
Lane 1 : SLC25A3/PHC transfected 293T cell line lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
ab89117, at 10 µg/ml staining SLC25A3/PHC in HeLa cells by Immunofluorescence.
ab89117 has not yet been referenced specifically in any publications.