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    slc25a6-antibody-ab153956.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-SLC25A6 antibody (ab153956)

  • Datasheet
  • SDS
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Western blot - Anti-SLC25A6 antibody (ab153956)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC25A6 antibody (ab153956)

Key features and details

  • Rabbit polyclonal to SLC25A6
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SLC25A6 antibody
    See all SLC25A6 primary antibodies
  • Description

    Rabbit polyclonal to SLC25A6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken, Cow, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 57-298 of Human SLC25A6 (Uniprot ID P12236).

  • Positive control

    • Recombinant Human SLC25A6 protein (ab152181) can be used as a positive control in WB. NCI-H929 whole cell lysate; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab153956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Sequence similarities

    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization

    Mitochondrion inner membrane. The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria.
  • Target information above from: UniProt accession P12236 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374072 Chicken
    • Entrez Gene: 282480 Cow
    • Entrez Gene: 293 Human
    • Entrez Gene: 403155 Pig
    • Entrez Gene: 566370 Zebrafish
    • Omim: 300151 Human
    • Omim: 403000 Human
    • SwissProt: P32007 Cow
    • SwissProt: P12236 Human
    • SwissProt: Q6QRN9 Pig
    • Unigene: 350927 Human
    see all
  • Alternative names

    • AAC3 antibody
    • Adenine nucleotide translocator 3 antibody
    • ADP antibody
    • ADP ATP carrier protein 3 antibody
    • ADP ATP carrier protein isoform T2 antibody
    • ADP ATP carrier protein liver antibody
    • ADP/ATP translocase 3 antibody
    • ADP/ATP translocator of liver antibody
    • ADT3_HUMAN antibody
    • ANT 2 antibody
    • ANT 3 antibody
    • ANT antibody
    • ANT3 antibody
    • ANT3Y antibody
    • ATP carrier protein 3 antibody
    • ATP carrier protein antibody
    • CDABP0051 antibody
    • isoform T2 antibody
    • MGC17525 antibody
    • SLC25A6 antibody
    • Solute carrier family 25 (mitochondrial carrier adenine nucleotide translocator) member 6 antibody
    • Solute carrier family 25 member 6 antibody
    see all

Images

  • Western blot - Anti-SLC25A6 antibody (ab153956)
    Western blot - Anti-SLC25A6 antibody (ab153956)
    Anti-SLC25A6 antibody (ab153956) at 1/1000 dilution + NCI-H929 whole cell lysate at 10 µg

    Predicted band size: 33 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC25A6 antibody (ab153956)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC25A6 antibody (ab153956)
    Immunofluorescence analysis of methanol-fixed HeLa, labeling SLC25A6 with ab153956 at 1/500 dilution. Blue: Hoechst 33342 staining.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab153956? Please let us know so that we can cite the reference in this datasheet.

    ab153956 has been referenced in 1 publication.

    • Lin C  et al. Caspase-Dependent Apoptosis Induction via Viral Protein ORF4 of Porcine Circovirus 2 Binding to Mitochondrial Adenine Nucleotide Translocase 3. J Virol 92:N/A (2018). PubMed: 29491154

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