Key features and details
- Rabbit polyclonal to SLC25A6
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC25A6 antibody
See all SLC25A6 primary antibodies
DescriptionRabbit polyclonal to SLC25A6
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- IHC-P: Human adipose tissue. ICC/IF: A549 cells. WB: A549 cell lysate. Flow Cyt: A549 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/10 - 1/50.|
|WB||1/1000. Predicted molecular weight: 33 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionCatalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Sequence similaritiesBelongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Cellular localizationMitochondrion inner membrane. The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria.
- Information by UniProt
- AAC3 antibody
- Adenine nucleotide translocator 3 antibody
- ADP antibody
Anti-SLC25A6 antibody (ab230545) at 1/1000 dilution + A549 (human lung carcinoma cell line) cell lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Formalin-fixed, paraffin-embedded human adipose tissue stained for SLC25A6 with ab230545 at 1/10 dilution in immunohistochemical analysis.
A549 (Human lung carcinoma cell line) cells stained for SLC25A6 using ab230545 at a dilution of 1/10 in ICC/IF, followed by AlexaFluor® 488-conjugated goat anti-rabbit lgG (green).
DAPI was used to stain the cell nucleus (blue).
Flow Cytometry analysis of A549 (Human lung carcinoma cell line) cells, labeling SLC25A6 with ab230545 at a 1/10 dilution (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230545 has not yet been referenced specifically in any publications.