Overview

  • Product name

    Anti-SLC26A6/Pat1 antibody
    See all SLC26A6/Pat1 primary antibodies
  • Description

    Goat polyclonal to SLC26A6/Pat1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SLC26A6/Pat1 (internal sequence). (NP_075062.2; NP_599025.2; NP_602298.2; NP_001035544.1).
    Sequence:

    C-SAPRTHQWRT

  • General notes


    This antibody is expected to recognize isoform 1, 2, 3 and 4 (NP_075062.2; NP_599025.2; NP_602298.2; NP_001035544.1).

     This product was previously labelled as SLC26A6

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Ammonium sulphate precipitation followed by antigen affinity chromatography.
  • Primary antibody notes

    This antibody is expected to recognize isoform 1, 2, 3 and 4 (NP_075062.2; NP_599025.2; NP_602298.2; NP_001035544.1).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 0.1 - 0.3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Does not seem to be involved in anion transport.
  • Tissue specificity

    Ubiquitous. Highest levels in kidney and pancreas. Lower expression in heart, skeletal muscle, liver and placenta. Also found in lung and brain.
  • Sequence similarities

    Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
    Contains 1 STAS domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anion exchange transporter antibody
    • Anion transporter 1 antibody
    • DKFZp586E1422 antibody
    • Pendrin L1 antibody
    • Pendrin like protein 1 antibody
    • Pendrin-L1 antibody
    • Pendrin-like protein 1 antibody
    • S26A6_HUMAN antibody
    • SLC26A6 antibody
    • Solute carrier family 26 member 6 antibody
    • Sulfate anion transporter antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue, staining SLC26A6/Pat1 with ab99559 at 0.1 &microg/ml. Antigen retrieval was performed by microwaving in a citrate buffer (pH 6). The image on the right was treated with the immunizing peptide.

  • All lanes : Anti-SLC26A6/Pat1 antibody (ab99559) at 10 µg/ml

    Lane 1 : Human renal cortical brush-border membrane lysate
    Lane 2 : Human renal cortical brush-border membrane lysate with immunizing peptide

    Lysates/proteins at 90 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Yin K  et al. SLC26A Gene Family Participate in pH Regulation during Enamel Maturation. PLoS One 10:e0144703 (2015). IHC-P ; Rat . Read more (PubMed: 26671068) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako retrieval solution (pH6)
Permeabilization
No
Specification
Intestine
Blocking step
(agent) for 10 minute(s) · Concentration: .25% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 14 2019

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