Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319-26]
    See all SLC27A4 / FATP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR17319-26] to SLC27A4 / FATP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SLC27A4/ FATP4 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6P1M0

  • Positive control

    • WB: HeLa, HepG2, HEK293, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human testis, fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. ICC/IF: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ICC/IF 1/100.

Target

  • Relevance

    SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • ACSVL 4 antibody
    • ACSVL4 antibody
    • EC 6.2.1 antibody
    • FATP 4 antibody
    • FATP4 antibody
    • Fatty acid transport protein 4 antibody
    • Fatty acid transport protein4 antibody
    • IPS antibody
    • Long chain fatty acid transport protein 4 antibody
    • Long chain fatty acid transport protein4 antibody
    • OTTHUMP00000022264 antibody
    • S27A4 antibody
    • SLC27 A4 antibody
    • SLC27A 4 antibody
    • Solute carrier family 27 (fatty acid transporter) member 4 antibody
    • Solute carrier family 27 member 4 antibody
    • Solute carrier family 27 member4 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SLC27A/FATP4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab200353 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab200353 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 4 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SLC27A4 / FATP4 with purified ab200353 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : Rat spleen lysate
    Lane 8 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 9 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 11 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 5 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30871020) »
See 1 Publication for this product

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