Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
![Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-395413-anti-slc27a4fatp4-antibody-epr17319-26-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17319-26] to SLC27A4 / FATP4
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SLC27A4 / FATP4 antibody [EPR17319-26]
See all SLC27A4 / FATP4 primary antibodies -
Description
Rabbit monoclonal [EPR17319-26] to SLC27A4 / FATP4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, HEK293, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human testis, fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17319-26 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
Target
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Relevance
SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 10999 Human
- Entrez Gene: 26569 Mouse
- Entrez Gene: 311839 Rat
- Omim: 604194 Human
- SwissProt: Q6P1M0 Human
- SwissProt: Q91VE0 Mouse
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Alternative names
- ACSVL 4 antibody
- ACSVL4 antibody
- EC 6.2.1 antibody
see all
Images
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Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SLC27A4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDaLanes 1-4: Merged signal (red and green). Green - ab200353 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.
ab200353 Anti-SLC27A4 / FATP4 antibody [EPR17319-26] was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-275844-anti-slc27a4-fatp4-antibody-epr17319-26-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SLC27A4 / FATP4 with purified ab200353 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-260191-anti-slc27a4-fatp4-antibody-epr17319-26-western-blot.jpg)
Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SLC27A/FATP4 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab200353 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab200353 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-242654-anti-slc27a4-fatp4-antibody-epr17319-26-western-blot.jpg)
Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 4 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-242653-anti-slc27a4-fatp4-antibody-epr17319-26-western-blot.jpg)
Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 30 secondsBlocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-242652-anti-slc27a4-fatp4-antibody-epr17319-26-western-blot.jpg)
Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat kidney lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cells) whole cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 11 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 5 secondsBlocking/dilution buffer: 5% NFDM/TBST.
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![Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)](https://www.abcam.com/ps/products/200/ab200353/Images/ab200353-9-benefits-of-recombinant-antibodies.png)
Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (6)
ab200353 has been referenced in 6 publications.
- Cheng L et al. Emodin Improves Glucose and Lipid Metabolism Disorders in Obese Mice via Activating Brown Adipose Tissue and Inducing Browning of White Adipose Tissue. Front Endocrinol (Lausanne) 12:618037 (2021). PubMed: 34040579
- Ibrahim A et al. Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport. Cell Metab 32:309-319.e7 (2020). PubMed: 32521232
- Supruniuk E et al. Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes. Int J Mol Sci 21:N/A (2020). PubMed: 33171690
- Søgaard D et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
- Dandanell S et al. Influence of maximal fat oxidation on long-term weight loss maintenance in humans. J Appl Physiol (1985) 123:267-274 (2017). PubMed: 28546468
- Zheng M et al. Immunohistochemical localization of fatty acid transporters and MCT1 in the sebaceous glands of mouse skin. Biomed Res 37:265-70 (2016). PubMed: 27545003
