Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

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Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Flow Cytometry - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
  • Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17319-26] to SLC27A4 / FATP4
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319-26]
    See all SLC27A4 / FATP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17319-26] to SLC27A4 / FATP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, HEK293, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human testis, fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Relevance

    SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • ACSVL 4 antibody
      • ACSVL4 antibody
      • EC 6.2.1 antibody
      • FATP 4 antibody
      • FATP4 antibody
      • Fatty acid transport protein 4 antibody
      • Fatty acid transport protein4 antibody
      • IPS antibody
      • Long chain fatty acid transport protein 4 antibody
      • Long chain fatty acid transport protein4 antibody
      • OTTHUMP00000022264 antibody
      • S27A4 antibody
      • SLC27 A4 antibody
      • SLC27A 4 antibody
      • Solute carrier family 27 (fatty acid transporter) member 4 antibody
      • Solute carrier family 27 member 4 antibody
      • Solute carrier family 27 member4 antibody
      see all

    Images

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : SLC27A4 knockout HEK293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HEK-293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab200353 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab200353 Anti-SLC27A4 / FATP4 antibody [EPR17319-26] was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Flow Cytometry - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

      Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SLC27A4 / FATP4 with purified ab200353 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SLC27A/FATP4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab200353 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab200353 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/5000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 3 : HEK293 (Human embryonic kidney) whole cell lysate
      Lane 4 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa


      Exposure time: 30 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : Rat brain lysate
      Lane 6 : Rat kidney lysate
      Lane 7 : Rat spleen lysate
      Lane 8 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 9 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 11 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa


      Exposure time: 5 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)
      Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353)

    References (3)

    Publishing research using ab200353? Please let us know so that we can cite the reference in this datasheet.

    ab200353 has been referenced in 3 publications.

    • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
    • Dandanell S  et al. Influence of maximal fat oxidation on long-term weight loss maintenance in humans. J Appl Physiol (1985) 123:267-274 (2017). PubMed: 28546468
    • Zheng M  et al. Immunohistochemical localization of fatty acid transporters and MCT1 in the sebaceous glands of mouse skin. Biomed Res 37:265-70 (2016). PubMed: 27545003

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