Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

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Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal
    See all SLC27A4 / FATP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human SLC27A4/ FATP4 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6P1M0

  • Positive control

    • WB: HeLa, HEK293 and HepG2 whole cell lysates. Human fetal brain and Human fetal kidney lysates. IHC-P: Human kidney and Mouse cardiac muscle tissue. ICC/IF: HepG2 cells. IP: HEK293 whole cell lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Relevance

    SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.

  • Cellular localization

    Cell Membrane

  • Database links

    see all

  • Alternative names

    • ACSVL 4 antibody
    • ACSVL4 antibody
    • EC 6.2.1 antibody
    • FATP 4 antibody
    • FATP4 antibody
    • Fatty acid transport protein 4 antibody
    • Fatty acid transport protein4 antibody
    • IPS antibody
    • Long chain fatty acid transport protein 4 antibody
    • Long chain fatty acid transport protein4 antibody
    • OTTHUMP00000022264 antibody
    • S27A4 antibody
    • SLC27 A4 antibody
    • SLC27A 4 antibody
    • Solute carrier family 27 (fatty acid transporter) member 4 antibody
    • Solute carrier family 27 member 4 antibody
    • Solute carrier family 27 member4 antibody
    see all

Images

  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SLC27A4/FATP4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab199719 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab199719 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: ab199719 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : HEK293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/2000 dilution

    Lane 1 : Human fetal brain
    Lane 2 : Human fetal kidney

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse cardiac muscle is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
    Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

    SLC27A4 / FATP4 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199719 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199719 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK293  whole cell lysate 10µg (Input). Lane 2: HEK293 whole cell lysate following IP. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199719 in HEK293 whole cell lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure is 3 minutes.

References

This product has been referenced in:

  • Yen MC  et al. Solute Carrier Family 27 Member 4 (SLC27A4) Enhances Cell Growth, Migration, and Invasion in Breast Cancer Cells. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30388870) »
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