Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - C-terminal
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal
    See all SLC27A4 / FATP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human SLC27A4/ FATP4 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6P1M0

  • Positive control

    • WB: HeLa, HEK293 and HepG2 whole cell lysates; Human fetal brain and Human fetal kidney lysates. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells. IP: HEK293 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Relevance

    SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • ACSVL 4 antibody
      • ACSVL4 antibody
      • EC 6.2.1 antibody
      • FATP 4 antibody
      • FATP4 antibody
      • Fatty acid transport protein 4 antibody
      • Fatty acid transport protein4 antibody
      • IPS antibody
      • Long chain fatty acid transport protein 4 antibody
      • Long chain fatty acid transport protein4 antibody
      • OTTHUMP00000022264 antibody
      • S27A4 antibody
      • SLC27 A4 antibody
      • SLC27A 4 antibody
      • Solute carrier family 27 (fatty acid transporter) member 4 antibody
      • Solute carrier family 27 member 4 antibody
      • Solute carrier family 27 member4 antibody
      see all

    Images

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : SLC27A4 knockout HEK293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HEK-293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab199719 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab199719 Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal  was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1: ab199719 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

      SLC27A4 / FATP4 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199719 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199719 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HEK293  whole cell lysate 10µg (Input). Lane 2: HEK293 whole cell lysate following IP. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199719 in HEK293 whole cell lysate.


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure is 3 minutes.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SLC27A4/FATP4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab199719 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab199719 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/10000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 3 : HEK293 (Human embryonic kidney) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719)
      All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/2000 dilution

      Lane 1 : Human fetal brain
      Lane 2 : Human fetal kidney

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

    References (6)

    Publishing research using ab199719? Please let us know so that we can cite the reference in this datasheet.

    ab199719 has been referenced in 6 publications.

    • Chassen SS  et al. Placental fatty acid transport across late gestation in a baboon model of intrauterine growth restriction. J Physiol 598:2469-2489 (2020). PubMed: 32338384
    • Shrestha N  et al. Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells. Cell Physiol Biochem 52:94-108 (2019). PubMed: 30790507
    • Wang H  et al. Pigment Epithelial-Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia. J Am Heart Assoc 8:e013028 (2019). PubMed: 31711388
    • Yen MC  et al. Solute Carrier Family 27 Member 4 (SLC27A4) Enhances Cell Growth, Migration, and Invasion in Breast Cancer Cells. Int J Mol Sci 19:N/A (2018). PubMed: 30388870
    • Gibbins KJ  et al. Effects of excess thromboxane A2 on placental development and nutrient transporters in a Mus musculus model of fetal growth restriction. Biol Reprod 98:695-704 (2018). PubMed: 29351577
    • Mauldin EA  et al. Cellular and Metabolic Basis for the Ichthyotic Phenotype in NIPAL4 (Ichthyin)-Deficient Canines. Am J Pathol 188:1419-1429 (2018). PubMed: 29548991

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