Key features and details
- Rabbit polyclonal to SLC27A5/BAL
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC27A5/BAL antibody
See all SLC27A5/BAL primary antibodies
DescriptionRabbit polyclonal to SLC27A5/BAL
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human SLC27A5/BAL (internal sequence).
- IHC-P: human liver carcinoma tissue. WB: extracts from HepG2 cells. ICC/IF: A549 cells.
Product previously known as SLC27A5.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab74869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 75 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/1000.|
FunctionAcyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
Tissue specificityPredominantly expressed in liver.
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- ACSB antibody
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All lanes : Anti-SLC27A5/BAL antibody (ab74869) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 75 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Immunohistochemistry analysis of SLC27A5/BAL in paraffin-embedded human liver carcinoma tissue using ab74869, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunofluorescence analysis of SLC27A5/BAL in A549 cells, using ab74869, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
ab74869 has been referenced in 1 publication.
- Przybylla S et al. Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches. PLoS One 11:e0159778 (2016). IP ; Human . PubMed: 27472061