Overview

  • Product name

    Anti-SLC31A1 / CTR1 antibody [EPR7936]
    See all SLC31A1 / CTR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7936] to SLC31A1 / CTR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SLC31A1/ CTR1 aa 1-100 (extracellular). The exact sequence is proprietary.
    (Peptide available as ab166648)

  • Positive control

    • HeLa, Human ovary cancer, Human fetal kidney and Caco 2 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.26 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7936
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28,35 kDa (predicted molecular weight: 21 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      High-affinity, saturable copper transporter involved in dietary copper uptake.
    • Sequence similarities

      Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
    • Post-translational
      modifications

      O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.
    • Cellular localization

      Cell membrane. Localizes to the apical membrane in intestinal epithelial cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Copper transport 1 homolog antibody
      • Copper transporter 1 antibody
      • COPT1 antibody
      • COPT1_HUMAN antibody
      • CTR1 antibody
      • hCTR1 antibody
      • High affinity copper uptake protein 1 antibody
      • SLC31A1 antibody
      • solute carrier family 31 (copper tansporters) member 1 antibody
      • Solute carrier family 31 member 1 antibody
      see all

    Images

    • All lanes : Anti-SLC31A1 / CTR1 antibody [EPR7936] (ab129067) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 21 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Fu C  et al. Active uptake of hydrophilic copper complex Cu(ii)-TETA in primary cultures of neonatal rat cardiomyocytes. Metallomics 11:565-575 (2019). Read more (PubMed: 30761393) »
    • Miller KA  et al. Antibiotic treatment affects the expression levels of copper transporters and the isotopic composition of copper in the colon of mice. Proc Natl Acad Sci U S A 116:5955-5960 (2019). Read more (PubMed: 30850515) »
    See all 12 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Neurons differentitated from iPS cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Neurons differentitated from iPS cells
    Blocking step
    Odyssey Blocking buffer from Li-Cor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 10 2017

    Answer

    The peptide ab166648 is the same immunogen which has been used to create the anti-SLC31A1/CTR1 antibody ab129067. This peptide fragement corresponds to a region of Human SLC31A1 / CTR1 within the first 30 amino acids.

    Read More

    Question
    Answer

    We do have the blocking peptide for ab129067 available through the company that we recently acquired, Epitomics. The peptide can be ordered through the epitomics link given below, and referencing P-5773. http://www.epitomics.com/products/product_info/1327/Blocking--P-XXXX.html
    Please note that the blocking peptide is not always available for each antibody. This is a part of the reason why Epitomics only has a generic page, rather than having the individual page for each blocking peptide.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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