Overview

  • Product name

    Anti-SLC31A1 / CTR1 antibody
    See all SLC31A1 / CTR1 primary antibodies
  • Description

    Rabbit polyclonal to SLC31A1 / CTR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide: MDHSHHMGMS YMDSNSTMQP SHHHPTTSAS HSHGGGDSSM MMMPMTFYFG , corresponding to N terminal amino acids 1-50 of Human SLC31A1/ CTR1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 21 kDa.
ELISA 1/500.

Target

  • Function

    High-affinity, saturable copper transporter involved in dietary copper uptake.
  • Sequence similarities

    Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
  • Post-translational
    modifications

    O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.
  • Cellular localization

    Cell membrane. Localizes to the apical membrane in intestinal epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Copper transport 1 homolog antibody
    • Copper transporter 1 antibody
    • COPT1 antibody
    • COPT1_HUMAN antibody
    • CTR1 antibody
    • hCTR1 antibody
    • High affinity copper uptake protein 1 antibody
    • SLC31A1 antibody
    • solute carrier family 31 (copper tansporters) member 1 antibody
    • Solute carrier family 31 member 1 antibody
    see all

References

This product has been referenced in:

  • Wang Q  et al. Gambogic acid reverses oxaliplatin resistance in colorectal cancer by increasing intracellular platinum levels. Oncol Lett 16:2366-2372 (2018). Read more (PubMed: 30008940) »
  • Galluzzi L  et al. Vitamin B6 metabolism influences the intracellular accumulation of cisplatin. Cell Cycle 12:417-21 (2013). Read more (PubMed: 23287530) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice  

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Answer

Thank you for your reply. If you would like, we can obtain the blocking peptide from the original laboratory. This is a product that you would have to purchase. If you are interested, I can add this product to our catalog for you to purchase from us. We are confident that the higher MW bands are aggregated products and we have seen them in cells transfected with CTR1 gene. The exact nature of aggregation is not clear but could represent covalent coupling as they still appear in reduced condition. Unfortunately, we are not able to provide a positive control. We would recommend intestinal epithelial cells. We've also tested the product in various ovarian cancer cell lines with positive results. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for contacting Abcam regarding ab108481. I am sorry that you are experiencing difficulties with this antibody in WB. I have reviewed your protocol information and the image of your blot. I have some suggestions that may explain your results. SLC31A1 is a protein that is glycosylated and therefore, higher molecular weight bands are expected. I am not sure about the glycosylation states in the cell lines you are examining, but I would recommend researching this further in the primary literature. I have attached an image showing the various bands recognized by this antibody due to glycosylation. At this time, this is the only antibody we have against this target and we are not able to generate one against a different portion of the protein. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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