Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC32A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Dog, Non human primates
  • Immunogen

    Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat SLC32A1. The amino acid sequence is not available.

  • Positive control
    • Rat hippocampal lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab42939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Involved in the uptake of GABA and glycine into the synaptic vesicles.
  • Tissue specificity
    Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
  • Sequence similarities
    Belongs to the amino acid/polyamine transporter 2 family.
  • Cellular localization
    Cytoplasmic vesicle membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA122O1.1 antibody
    • GABA and glycine transporter antibody
    • hVIAAT antibody
    • SLC32A 1 antibody
    • Slc32a1 antibody
    • solute carrier family 32 (GABA vesicular transporter) member 1 antibody
    • Solute carrier family 32 member 1 antibody
    • Vesicular GABA Amino Acid Transporter antibody
    • Vesicular GABA transporter antibody
    • Vesicular inhibitory amino acid transporter antibody
    • VGAT antibody
    • VIAAT antibody
    • VIAAT_HUMAN antibody
    see all

Images

  • Anti-SLC32A1 antibody (ab42939) at 1/1000 dilution + Rat hippocampal lysate at 20 µg

    Secondary
    Goat-anti-rabbit HRP. at 1/15000 dilution

    Predicted band size: 58 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing (4-12% gel)
Loading amount
25 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 04 2018

Question
Answer

Thank you for contacting Abcam.

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for the update. It may be that this antibody is incapable of detecting the rat protein. Do you have any human samples you could run as a positive control?

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Question
Answer

I have received feedback from the source of our VGAT antibody reference ab42939 and was informed that there is 92 % homology of the immunogen with the mouse sequence therefore it may work in this species but currently we cannot guarantee it as it has not been tested. As soon as I have received information about our VGLUT2 antibody I will contact you, thank you for your patience,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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