Anti-SLC34A1 antibody (ab151129)
Key features and details
- Rabbit polyclonal to SLC34A1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC34A1 antibody -
Description
Rabbit polyclonal to SLC34A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Recombinant fragment, corresponding to amino acids 15-97 of Human SLC34A1 (UniProt: Q06495).
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Positive control
- IHC-P: Human kidney tissue. WB: Human kidney lysate. ICC/IF: HEL cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.
|
| Notes |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa. |
Target
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Relevance
May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 6569 Human
- Omim: 182309 Human
- SwissProt: Q06495 Human
- Unigene: 936 Human
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Alternative names
- FRTS2 antibody
- Na(+)-dependent phosphate cotransporter 2A antibody
- Na(+)/Pi cotransporter 2A antibody
see all
Images
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Western blot - Anti-SLC34A1 antibody (ab151129)Lane 2 : Anti-SLC34A1 antibody (ab151129)
Lane 1 : Marker
Lane 2 : Human kidney tissue lysate
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)Immunohistochemical analysis of Formalin fixed Paraffin embedded human kidney tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SLC34A1 antibody (ab151129)HEL cells stained for SLC34A1 (green) using ab151129 at 2 µg/ml dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)Immunohistochemical analysis of Formalin fixed Paraffin embedded human prostate tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
No positivity in glandular cells as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)Immunohistochemical analysis of Formalin fixed Paraffin embedded human stomach tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
No positivity in glandular cells as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)Immunohistochemical analysis of Formalin fixed Paraffin embedded human placenta tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
No positivity in trophoblastic cells as expected.
Protocols
Datasheets and documents
References (1)
ab151129 has been referenced in 1 publication.
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927
