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    slc34a1-antibody-ab151129.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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Anti-SLC34A1 antibody (ab151129)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-SLC34A1 antibody (ab151129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC34A1 antibody (ab151129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)

Key features and details

  • Rabbit polyclonal to SLC34A1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SLC34A1 antibody
  • Description

    Rabbit polyclonal to SLC34A1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 15-97 of Human SLC34A1 (UniProt: Q06495).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human kidney tissue. WB: Human kidney lysate. ICC/IF: HEL cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Application Abreviews Notes
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.
Notes
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.

Target

  • Relevance

    May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx.
  • Cellular localization

    Membrane; Multi-pass membrane protein.
  • Database links

    • Entrez Gene: 6569 Human
    • Omim: 182309 Human
    • SwissProt: Q06495 Human
    • Unigene: 936 Human
    • Alternative names

      • FRTS2 antibody
      • Na(+)-dependent phosphate cotransporter 2A antibody
      • Na(+)/Pi cotransporter 2A antibody
      • Na+-phosphate cotransporter type II antibody
      • naPi-2a antibody
      • NAPI-3 antibody
      • naPi2a antibody
      • NAPI3 antibody
      • NPHLOP1 antibody
      • NPT2 antibody
      • NPTIIa antibody
      • renal sodium-dependent phosphate transporter antibody
      • renal sodium/phosphate transporter protein 2 antibody
      • SLC11 antibody
      • SLC17A2 antibody
      • SLC17A2, formerly antibody
      • sodium-dependent phosphate transport protein 2A antibody
      • sodium-phosphate transport protein 2A antibody
      • sodium/phosphate co-transporter antibody
      • sodium/phosphate cotransporter 2A antibody
      • sodium/phosphate transporter 2, kidney antibody
      • solute carrier family 17 (sodium phosphate), member 2 antibody
      • solute carrier family 17, member 2, formerly antibody
      • solute carrier family 34 (sodium phosphate), member 1 antibody
      • solute carrier family 34 (type II sodium/phosphate contransporter), member 1 antibody
      • solute carrier family 34 member 1 antibody
      see all

    Images

    • Western blot - Anti-SLC34A1 antibody (ab151129)
      Western blot - Anti-SLC34A1 antibody (ab151129)
      Lane 2 : Anti-SLC34A1 antibody (ab151129)

      Lane 1 : Marker
      Lane 2 : Human kidney tissue lysate

      Predicted band size: 69 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)

      Immunohistochemical analysis of Formalin fixed Paraffin embedded human kidney tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC34A1 antibody (ab151129)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC34A1 antibody (ab151129)

      HEL cells stained for SLC34A1 (green) using ab151129 at 2 µg/ml dilution in ICC/IF. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)

      Immunohistochemical analysis of Formalin fixed Paraffin embedded human prostate tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      No positivity in glandular cells as expected.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)

      Immunohistochemical analysis of Formalin fixed Paraffin embedded human stomach tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      No positivity in glandular cells as expected.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC34A1 antibody (ab151129)

      Immunohistochemical analysis of Formalin fixed Paraffin embedded human placenta tissue labeling SLC34A1 with ab151129 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      No positivity in trophoblastic cells as expected.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab151129? Please let us know so that we can cite the reference in this datasheet.

    ab151129 has been referenced in 1 publication.

    • Castella B  et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927

    Customer reviews and Q&As

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