Recombinant
RabMAb

Recombinant Anti-SLC34A2 antibody [SP322] - BSA and Azide free (ab238793)

Overview

  • Product name

    Anti-SLC34A2 antibody [SP322] - BSA and Azide free
    See all SLC34A2 primary antibodies
  • Description

    Rabbit monoclonal [SP322] to SLC34A2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC34A2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95436

  • Positive control

    • IHC-P: Human lung adenocarcinoma and endometrial carcinoma tissues ICC/IF: NIH/OVCAR-3 cells FC: OvCAR-3 cells
  • General notes

    Ab238793 is the carrier-free version of ab228474. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238793 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab238793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.
  • Tissue specificity

    Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium.
  • Involvement in disease

    Defects in SLC34A2 are a cause of pulmonary alveolar microlithiasis (PALM) [MIM:265100]. Pulmonary alveolar microlithiasis is a rare disease characterized by the deposition of calcium phosphate microliths throughout the lungs. Most patients are asymptomatic for several years or even for decades and generally, the diagnosis is incidental to clinical investigations unrelated to the disease. Cases with early onset or rapid progression are rare. A 'sandstorm-appearing' chest roentgenogram is a typical diagnostic finding. The onset of this potentially lethal disease varies from the neonatal period to old age and the disease follows a long-term, progressive course, resulting in a slow deterioration of lung functions. Pulmonary alveolar microlithiasis is a recessive monogenic disease with full penetrance.
  • Sequence similarities

    Belongs to the SLC34A transporter family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Na(+)-dependent phosphate cotransporter 2B antibody
    • Na(+)/Pi cotransporter 2B antibody
    • NaPi-2b antibody
    • NaPi3b antibody
    • NPT2B_HUMAN antibody
    • SLC34A2 antibody
    • Sodium-dependent phosphate transport protein 2B antibody
    • Sodium-phosphate transport protein 2B antibody
    • Sodium/phosphate cotransporter 2B antibody
    • Solute carrier family 34 member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the luminal surface of human endometrial carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228474 for 10 mins at room temperature. This image was generated using ab228474, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SLC34A2 with ab228474 at 1/100 dilution (2.37 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228474 for 10 mins at room temperature. This image was generated using ab228474, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence analysis of NIH/OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labeling SLC34A2 with purified ab228474 at 1/100 (2.4 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Flow cytometry analysis of OvCAR-3 (human ovary adenocarcinoma) labeling SLC34A2 with purified ab228474 at 1/20 dilution (11.85 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

  • Formalin-fixed, paraffin-embedded human uterus tissue stained for SLC34A2 using ab228474 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228474).

References

ab238793 has not yet been referenced specifically in any publications.

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