Anti-SLC35A2 antibody (ab222854)
Key features and details
- Rabbit polyclonal to SLC35A2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC35A2 antibody -
Description
Rabbit polyclonal to SLC35A2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog -
Immunogen
Recombinant fragment corresponding to Human SLC35A2 aa 100-250.
Database link: P78381 -
Positive control
- WB: Mouse stomach tissue lysate. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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WB |
1/2000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/2000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa). |
Target
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Cellular localization
Golgi apparatus membrane; Multi-pass membrane protein -
Database links
- Entrez Gene: 338042 Cow
- Entrez Gene: 403595 Dog
- Entrez Gene: 7355 Human
- Entrez Gene: 22232 Mouse
- Omim: 314375 Human
- SwissProt: Q58DA6 Cow
- SwissProt: Q8WMS0 Dog
- SwissProt: P78381 Human
see all -
Alternative names
- Solute carrier family 35 (UDP galactose transporter) member A2 antibody
- Solute carrier family 35 member A2 antibody
- UDP Gal Tr antibody
see all
Images
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Anti-SLC35A2 antibody (ab222854) at 1/2000 dilution + Mouse stomach tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
PFA-fixed, Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SLC35A2 (green) using ab222854 at 1/100 dilution in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab222854 has been referenced in 2 publications.
- Liu CL et al. Aberrant Expression of Solute Carrier Family 35 Member A2 Correlates With Tumor Progression in Breast Cancer. In Vivo 37:262-269 (2023). PubMed: 36593004
- Fu Y et al. Legionella pneumophila Infection of Human Macrophages Retains Golgi Structure but Reduces O-Glycans. Pathogens 11:N/A (2022). PubMed: 36015029