Key features and details
- Rabbit polyclonal to SLC36A1 - N-terminal
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SLC36A1 antibody - N-terminal
DescriptionRabbit polyclonal to SLC36A1 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
- Human kidney tissue. NCI-460 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 53 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
FunctionNeutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid.
Sequence similaritiesBelongs to the amino acid/polyamine transporter 2 family.
Cellular localizationCell membrane. Lysosome membrane.
- Information by UniProt
- Dct1 antibody
- hPAT1 antibody
- LYAAT1 antibody
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney sections labeling SLC36A1 using ab189441 at 5 µg/ml.
Immunofluorescent staining of NCI-460 cells labeling SLC36A1 using ab189441 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-460 cells (right histogram) compared to a negative control cell (left histogram) labeling SLC36A1 using ab189441 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
ab189441 has not yet been referenced specifically in any publications.